Journal: Biomaterials Research
Article Title: Thermo-responsive Bioink for Personalized 3D Printed Scaffolds with Antioxidant and Fibroblast Delivery to Accelerate Diabetic Wound Healing
doi: 10.34133/bmr.0216
Figure Lengend Snippet: Histological analysis of skin tissues after 14 d of treatment with TP, PC, and PCC bioinks. (A) Hematoxylin and eosin (H&E) staining (×40; scale bar: 50 μm) and Masson’s trichrome (MT) staining (×20; scale bar: 50 μm) and wound lengths, thicknesses, epidermal thicknesses, and collagen deposition ( n = 4 each group). (B) Immunohistochemical (IHC) staining of CD206 in wound tissues on treatment day 14 ( n = 4 each group; scale bar: 100 μm). (C) Immunofluorescence (IF) images of CD31 and alpha-smooth muscle actin (α-SMA) in wound tissues on treatment day 14 (scale bar: 100 μm). Quantitation was performed using the ImageJ software, and results are expressed as mean ± standard deviation. DAPI, 4′,6-diamidino-2-phenylindole.
Article Snippet: Hematoxylin staining (H-3404, Vector Laboratories, USA) was then performed, and the slides were mounted with mounting solution (H-5501, Vector Laboratories, USA).
Techniques: Staining, Immunohistochemical staining, Immunohistochemistry, Immunofluorescence, Quantitation Assay, Software, Standard Deviation